|
|
| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
|
Biblioteca (s) : |
INIA La Estanzuela; INIA Las Brujas; INIA Tacuarembó. |
Fecha : |
16/10/2014 |
Actualizado : |
30/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
PÉREZ, C.A.; WINGFIELD, M.J.; ALTIER, N.; SIMETO, S.; BLANCHETTE, R.A. |
Afiliación : |
NORA ADRIANA ALTIER MANZINI, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; SOFIA SIMETO FERRARI, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
Título : |
Puccinia psidii infecting cultivated Eucalyptus and native myrtaceae in Uruguay. |
Fecha de publicación : |
2011 |
Fuente / Imprenta : |
Mycological Progress, 2011, v.10, no. 3, p. 273-282. |
DOI : |
10.1007/s11557-010-0698-x |
Idioma : |
Inglés |
Notas : |
Article history: Received: 2 June 2010 // Revised: 13 July 2010 //Accepted: 19 July 2010 // Published online: 12 August 2010. |
Contenido : |
ABSTRACT.
Eucalyptus or guava rust caused by Puccinia psidii is a serious disease of Eucalyptus and other Myrtaceae. In Uruguay, it has been previously found on Eucalyptus globulus and Psidium brasiliensis. Almost nothing is known regarding the occurrence of this pathogen on other Eucalyptus species or native Myrtaceae in that country. In this study, we determined the presence of P. psidii on Eucalyptus species and native Myrtaceae trees in Uruguay and evaluated the pathogenicity of specimens from native myrtaceous hosts on E. globulus and E. grandis. Phylogenetic analyses based on the internal transcribed spacer (ITS) region of the nuclear ribosomal DNA operon were used to confirm pathogen identity. Comparisons of ITS sequences confirmed the identity of P. psidii on Eucalyptus globulus, E. grandis, Myrcianthes pungens, and Myrrhinium atropurpureum var. octandrum. This is the first report of P. psidii on M. atropurpureum var. octandrum. Pathogenicity tests showed that isolates from native Myrtaceae could infect both Eucalyptus species tested, indicating a strong biological relationship between both introduced and native Myrtaceae. This study supplies relevant field data, morphological information, molecular phylogenetic analyses and infection studies that contribute to a better understanding of an important and little studied pathogen.
© 2010 German Mycological Society and Springer. |
Palabras claves : |
PUCCINIA PSIDIS. |
Thesagro : |
ENFERMEDADES FORESTALES; EUCALYPTUS; EUCALYPTUS GLOBULUS. |
Asunto categoría : |
-- H20 Enfermedades de las plantas |
Marc : |
LEADER 02206naa a2200241 a 4500 001 1012842 005 2019-10-30 008 2011 bl uuuu u00u1 u #d 024 7 $a10.1007/s11557-010-0698-x$2DOI 100 1 $aPÉREZ, C.A. 245 $aPuccinia psidii infecting cultivated Eucalyptus and native myrtaceae in Uruguay. 260 $c2011 500 $aArticle history: Received: 2 June 2010 // Revised: 13 July 2010 //Accepted: 19 July 2010 // Published online: 12 August 2010. 520 $aABSTRACT. Eucalyptus or guava rust caused by Puccinia psidii is a serious disease of Eucalyptus and other Myrtaceae. In Uruguay, it has been previously found on Eucalyptus globulus and Psidium brasiliensis. Almost nothing is known regarding the occurrence of this pathogen on other Eucalyptus species or native Myrtaceae in that country. In this study, we determined the presence of P. psidii on Eucalyptus species and native Myrtaceae trees in Uruguay and evaluated the pathogenicity of specimens from native myrtaceous hosts on E. globulus and E. grandis. Phylogenetic analyses based on the internal transcribed spacer (ITS) region of the nuclear ribosomal DNA operon were used to confirm pathogen identity. Comparisons of ITS sequences confirmed the identity of P. psidii on Eucalyptus globulus, E. grandis, Myrcianthes pungens, and Myrrhinium atropurpureum var. octandrum. This is the first report of P. psidii on M. atropurpureum var. octandrum. Pathogenicity tests showed that isolates from native Myrtaceae could infect both Eucalyptus species tested, indicating a strong biological relationship between both introduced and native Myrtaceae. This study supplies relevant field data, morphological information, molecular phylogenetic analyses and infection studies that contribute to a better understanding of an important and little studied pathogen. © 2010 German Mycological Society and Springer. 650 $aENFERMEDADES FORESTALES 650 $aEUCALYPTUS 650 $aEUCALYPTUS GLOBULUS 653 $aPUCCINIA PSIDIS 700 1 $aWINGFIELD, M.J. 700 1 $aALTIER, N. 700 1 $aSIMETO, S. 700 1 $aBLANCHETTE, R.A. 773 $tMycological Progress, 2011$gv.10, no. 3, p. 273-282.
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA Las Brujas (LB) |
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
| Acceso al texto completo restringido a Biblioteca INIA Treinta y Tres. Por información adicional contacte bibliott@inia.org.uy. |
Registro completo
|
Biblioteca (s) : |
INIA Treinta y Tres. |
Fecha actual : |
28/03/2022 |
Actualizado : |
02/12/2022 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
DE ARMAS, S.; GALVÁN, G. A.; LAPAZ, M. I.; GONZÁLEZ-BARRIOS, P.; VICENTE, E.; PIANZZOLA, M. J.; SIRI, M. I. |
Afiliación : |
STEFANIE DE ARMAS, Área Microbiología, Departamento de Biociencias, Facultad de Química, UDELAR, Montevideo, UY.; Programa de Desarrollo de las Ciencias Básicas (PEDECIBA).; GUILLERMO A. GALVÁN, Departamento de Producción Vegetal, Centro Regional Sur (CRS), Facultad de Agronomía, UDELAR, Canelones, UY. Programa de Desarrollo de las Ciencias Básicas (PEDECIBA).; MARÍA I. LAPAZ, Área Microbiología, Departamento de Biociencias, Facultad de Química, UDELAR, Montevideo, uy. Programa de Desarrollo de las Ciencias Básicas (PEDECIBA).; PABLO GONZÁLEZ-BARRIOS, Departamento de Biometría, Estadística y Computación. Facultad de Agronomía, UDELAR, Montevideo, UY.; CARLOS ESTEBAN VICENTE CASTRO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA J. PIANZZOLA, Área Microbiología, Departamento de Biociencias, Facultad de Química, UDELAR, Montevideo, UY. Programa de Desarrollo de las Ciencias Básicas (PEDECIBA).; MARÍA I. SIRI, Área Microbiología, Departamento de Biociencias, Facultad de Química, UDELAR, Montevideo, UY. Programa de Desarrollo de las Ciencias Básicas (PEDECIBA). |
Título : |
Phylogeny and identification of Pantoea species associated with bulb rot and bacterial leaf blight of onion crops in Uruguay. |
Fecha de publicación : |
2022 |
Fuente / Imprenta : |
Plant Disease, 2022, volume 106, issue 4, pp. 1216-1225. doi: https://doi.org/10.1094/PDIS-06-21-1140-RE |
DOI : |
10.1094/PDIS-06-21-1140-RE |
Idioma : |
Inglés |
Notas : |
Published Online:27 Mar 2022.
Corresponding author: María I. Siri; Email: msiri@fq.edu.uy. |
Contenido : |
Abstract
Onion is among the most consumed vegetables in Uruguay, grown in the northwestern and southern regions of the country. The onion supply presents interannual variations associated with significant postharvest losses, mainly caused by bacterial rots. Besides bulb rotting, onion leaf lesions as well as infections on seed-stalks during seed production may be devastating for some varieties under conducive conditions. This research aimed to identify the causal agents of bulb rots and leaf blight of onion crops in Uruguay. Symptomatic bulbs, seeds-stalks, and leaves were collected from commercial fields from 2015 to 2020. Bacterial colonies were isolated and identified at genera level using physiological tests and 16S rRNA gene sequence analysis. A collection of 59 Pantoea spp. isolates was obtained (11 from bulbs and 48 from leaves and seeds-stalks). Multilocus sequence analysis using four housekeeping genes (rpoB, gyrB, leuS, and fusA) allowed the assignment of the isolates to five Pantoea species: P. ananatis, P. agglomerans, P. allii, P. eucalypti, and P. vagans. The last two species were not previously reported as onion pathogens elsewhere. The ability to cause disease symptoms was tested by leaf inoculation and red onion scale assays. P. ananatis isolates showed the highest aggressiveness in both assays. Specific isolates from P. allii (MAI 6022), P. eucalypti (MAI 6036), P. vagans (MAI 6050), and Pantoea sp. (MAI 6049) ranked second in aggressiveness on onion leaves, whereas only three isolates belonging to P. eucalypti (MAI 6036 and MAI 6058) and P. agglomerans (MAI 6045) exhibited the same scale-clearing phenotype as P. ananatis. Leaf inoculation assays were also performed on a set of eight onion cultivars and breeding lines. Overall, P. ananatis MAI 6032 showed the highest aggressiveness in all tested cultivars, followed by P. eucalypti MAI 6036. The presence of new reported bacterial species leads to complex disease management and highlights the need for further studies on virulence factors and the epidemiology of these pathogens. MenosAbstract
Onion is among the most consumed vegetables in Uruguay, grown in the northwestern and southern regions of the country. The onion supply presents interannual variations associated with significant postharvest losses, mainly caused by bacterial rots. Besides bulb rotting, onion leaf lesions as well as infections on seed-stalks during seed production may be devastating for some varieties under conducive conditions. This research aimed to identify the causal agents of bulb rots and leaf blight of onion crops in Uruguay. Symptomatic bulbs, seeds-stalks, and leaves were collected from commercial fields from 2015 to 2020. Bacterial colonies were isolated and identified at genera level using physiological tests and 16S rRNA gene sequence analysis. A collection of 59 Pantoea spp. isolates was obtained (11 from bulbs and 48 from leaves and seeds-stalks). Multilocus sequence analysis using four housekeeping genes (rpoB, gyrB, leuS, and fusA) allowed the assignment of the isolates to five Pantoea species: P. ananatis, P. agglomerans, P. allii, P. eucalypti, and P. vagans. The last two species were not previously reported as onion pathogens elsewhere. The ability to cause disease symptoms was tested by leaf inoculation and red onion scale assays. P. ananatis isolates showed the highest aggressiveness in both assays. Specific isolates from P. allii (MAI 6022), P. eucalypti (MAI 6036), P. vagans (MAI 6050), and Pantoea sp. (MAI 6049) ranked second in aggressiveness on onion leaves... Presentar Todo |
Palabras claves : |
ALLIUM CEPA; CENTER ROT; PANTOEA SPP; PATHOGEN DIVERSITY. |
Asunto categoría : |
-- |
Marc : |
LEADER 03015naa a2200265 a 4500 001 1062926 005 2022-12-02 008 2022 bl uuuu u00u1 u #d 024 7 $a10.1094/PDIS-06-21-1140-RE$2DOI 100 1 $aDE ARMAS, S. 245 $aPhylogeny and identification of Pantoea species associated with bulb rot and bacterial leaf blight of onion crops in Uruguay.$h[electronic resource] 260 $c2022 500 $aPublished Online:27 Mar 2022. Corresponding author: María I. Siri; Email: msiri@fq.edu.uy. 520 $aAbstract Onion is among the most consumed vegetables in Uruguay, grown in the northwestern and southern regions of the country. The onion supply presents interannual variations associated with significant postharvest losses, mainly caused by bacterial rots. Besides bulb rotting, onion leaf lesions as well as infections on seed-stalks during seed production may be devastating for some varieties under conducive conditions. This research aimed to identify the causal agents of bulb rots and leaf blight of onion crops in Uruguay. Symptomatic bulbs, seeds-stalks, and leaves were collected from commercial fields from 2015 to 2020. Bacterial colonies were isolated and identified at genera level using physiological tests and 16S rRNA gene sequence analysis. A collection of 59 Pantoea spp. isolates was obtained (11 from bulbs and 48 from leaves and seeds-stalks). Multilocus sequence analysis using four housekeeping genes (rpoB, gyrB, leuS, and fusA) allowed the assignment of the isolates to five Pantoea species: P. ananatis, P. agglomerans, P. allii, P. eucalypti, and P. vagans. The last two species were not previously reported as onion pathogens elsewhere. The ability to cause disease symptoms was tested by leaf inoculation and red onion scale assays. P. ananatis isolates showed the highest aggressiveness in both assays. Specific isolates from P. allii (MAI 6022), P. eucalypti (MAI 6036), P. vagans (MAI 6050), and Pantoea sp. (MAI 6049) ranked second in aggressiveness on onion leaves, whereas only three isolates belonging to P. eucalypti (MAI 6036 and MAI 6058) and P. agglomerans (MAI 6045) exhibited the same scale-clearing phenotype as P. ananatis. Leaf inoculation assays were also performed on a set of eight onion cultivars and breeding lines. Overall, P. ananatis MAI 6032 showed the highest aggressiveness in all tested cultivars, followed by P. eucalypti MAI 6036. The presence of new reported bacterial species leads to complex disease management and highlights the need for further studies on virulence factors and the epidemiology of these pathogens. 653 $aALLIUM CEPA 653 $aCENTER ROT 653 $aPANTOEA SPP 653 $aPATHOGEN DIVERSITY 700 1 $aGALVÁN, G. A. 700 1 $aLAPAZ, M. I. 700 1 $aGONZÁLEZ-BARRIOS, P. 700 1 $aVICENTE, E. 700 1 $aPIANZZOLA, M. J. 700 1 $aSIRI, M. I. 773 $tPlant Disease, 2022, volume 106, issue 4, pp. 1216-1225. doi: https://doi.org/10.1094/PDIS-06-21-1140-RE
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA Treinta y Tres (TT) |
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
Expresión de búsqueda válido. Check! |
|
|